American Society of Microbiology ABMM

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American Society of Microbiology ABMM Sample Questions:

1. An immunocompromised patient develops a disseminated fungal infection. Blood cultures are consistently negative. However, a serum beta-D-glucan assay is markedly elevated. Tissue biopsy reveals the presence of hyaline, septate hyphae with dichotomous branching. The MOST likely causative organism is:

A) Mucor species
B) Rhizopus species
C) Aspergillus fumigatus
D) Cryptococcus neoformans

2. A clinical laboratory manager is implementing a new automated blood culture system. Which aspect is LEAST likely to be part of the initial verification/validation process before clinical use?

A) Comparison of time-to-detection with the previous system
B) Verification of detection of a panel of common bloodstream pathogens
C) Establishing workflow and training competency for laboratory staff
D) Assessment of the system's contamination rate over one year of routine use

3. An immunocompromised patient returning from a trip to the southwestern United States presents with fever, cough, and chest pain. Chest X-ray shows diffuse pulmonary infiltrates. Sputum culture grows a dimorphic fungus that forms spherules containing endospores at 37°C. The MOST likely causative agent is:

A) Histoplasma capsulatum
B) Coccidioides immitis
C) Paracoccidioides brasiliensis
D) Blastomyces dermatitidis

4. A research team is investigating the host immune response to Mycobacterium tuberculosis. They discover that a specific mycobacterial glycolipid inhibits the maturation of phagosomes containing the bacteria, preventing their fusion with lysosomes. This mechanism allows M. tuberculosis to:

A) Survive and replicate within macrophages.
B) Evade detection by Toll-like receptors on macrophages.
C) Escape from the phagolysosome into the cytoplasm.
D) Induce the production of pro-inflammatory cytokines.

5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:

A) Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
B) Modification of the peptidoglycan structure, preventing penicillin binding.
C) Increased activity of efflux pumps that actively remove penicillin from the cell.
D) Production of beta-lactamase enzymes that hydrolyze penicillin.

Solutions: